Subsequently, gene and protein expression changes associated with the upregulation, downregulation, and irradiation of CCNDBP1 were assessed. Hepatocellular carcinoma (HCC) cells and tissues obtained from Ccndbp1 knockout mice were used for the in vitro and in vivo examination of the molecular mechanisms of CCNDBP1 associated with the recovery of cells from DNA damage. Therefore, this study focused on investigating the function of CCNDBP1, which is directly related to the survival of cancer cells by escaping DNA damage and chemoresistance. GAPDH as the control *P < 0.05 **P < 0.01 ***P < 0.001 ****P < 0.0001.Ĭyclin D1 binding protein 1 (CCNDBP1) is considered a tumor suppressor, and when expressed in tumor cells, CCNDBP1 can contribute to the viability of cancer cells by rescuing these cells from chemotherapy-induced DNA damage. In addition, the expression of TCF-8, b-catenin, Snail, and Slug, transcription factors related to the pathological EMT, were downregulated. The expression of E-cadherin, ZO-1, and Claudin-1 proteins related to epithelial cell structures and tight junctions, was upregulated, while the expression of N-cadherin and Vimentin markers related to the mesenchymal cell status was downregulated. The expressions of TCF-8, b-catenin, Snail, and Slug, which were transcription factors related with pathological EMT, were apparently upregulated (E) The expression levels of EMT-related markers were significantly changed after CCNDBP1 overexpression in LPS510 cells. The expressions of E-cadherin, ZO-1, and Claudin-1, proteins related with epithelial cell structures and tight junctions, were apparently downregulated and the expressions of N-cadherin and Vimentin, markers related with mesenchymal cell status, were apparently upregulated.

Clusters with a statistical significance (FDR q < 0.05) were mainly marked (C) The result subset of GSEA, mainly involving the EMT, E2F targets, and G2M checkpoints (D) Based on the Western blot experiments, the expression levels of the EMT-related markers were significantly changed after the interference of CCNDBP1 expression in LPS853 cells. NC as the control, red for upregulation and blue for downregulation (B) Gene function annotation cluster of the GSEA Hallmarks. (A) The heatmap of gene expression after the interference of CCNDBP1 expression by siRNA in LPS853 cells. | CCNDBP1 could regulate the malignant potentials of DDL cells by repressing the pathological EMT.

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